MARC details
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04238cam a2200697Ia 4500 |
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ocn264389562 |
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OCoLC |
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20200709135927.0 |
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m o d |
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cr cn||||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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081029s2008 gw a ob 001 0 eng d |
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DG1 |
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eng |
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pn |
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DG1 |
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DG1 |
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YDXCP |
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BTCTA |
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M6U |
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DG1 |
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OCLCO |
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MERUC |
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OCLCQ |
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DEBBG |
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259259695 |
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277256524 |
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294884396 |
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536380071 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9783527622290 |
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| International Standard Book Number |
3527622292 |
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| International Standard Book Number |
9783527622306 |
| Qualifying information |
(electronic bk.) |
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| International Standard Book Number |
3527622306 |
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(electronic bk.) |
| 024 7# - |
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10.1002/9783527622290 |
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doi |
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AU@ |
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000046755708 |
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DEBSZ |
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430812868 |
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DEBSZ |
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452487471 |
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592830977 |
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DEBBG |
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BV043388763 |
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(OCoLC)264389562 |
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(OCoLC)259259695 |
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(OCoLC)277256524 |
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(OCoLC)294884396 |
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(OCoLC)536380071 |
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9783527622290 |
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Wiley InterScience |
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http://www3.interscience.wiley.com |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
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QP551 |
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.P75673 2008eb |
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2008 E-811 |
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QU 25 |
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P9683 2008 |
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SCI |
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007000 |
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bisacsh |
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| Classification number |
572.64 |
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22 |
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MAIN |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Westermeier, Reiner. |
| 245 10 - TITLE STATEMENT |
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| Title |
Proteomics in practice : |
| Remainder of title |
a guide to successful experimental design / |
| Statement of responsibility, etc |
Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker. |
| 250 ## - EDITION STATEMENT |
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| Edition statement |
2nd ed. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
1 online resource (xx, 482 pages) : |
| Other physical details |
illustrations (some color) |
| 500 ## - GENERAL NOTE |
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| General note |
Previous edition: 2002. |
| 505 0# - |
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| Formatted contents note |
Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- pt. III. Trouble shooting -- Glossary of terms. |
| 650 #0 - |
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| Topical term or geographic name as entry element |
Proteins |
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| Topical term or geographic name as entry element |
Molecular biology. |
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| Topical term or geographic name as entry element |
Proteome. |
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| Topical term or geographic name as entry element |
Proteomics. |
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| Topical term or geographic name as entry element |
SCIENCE |
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| Topical term or geographic name as entry element |
Molecular biology. |
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| Topical term or geographic name as entry element |
Proteins |
| 700 1# - |
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| Personal name |
Naven, Tom. |
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| Personal name |
Höpker, Hans-Rudolf. |
| 710 2# - |
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| Corporate name or jurisdiction name as entry element |
Wiley InterScience (Online service) |
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| Uniform Resource Identifier |
https://doi.org/10.1002/9783527622290 |
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Weinheim : |
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Wiley-VCH ; |
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2008. |
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Chichester : |
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John Wiley [distributor], |
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2008. |
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text |
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txt |
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rdacontent |
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computer |
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c |
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rdamedia |
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online resource |
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cr |
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rdacarrier |
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Includes bibliographical references and index. |
| 520 ## - |
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A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third sectio. |
| 588 0# - |
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Print version record. |
| 650 #0 - |
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Analysis. |
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Life Sciences |
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Biochemistry. |
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bisacsh |
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fast |
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(OCoLC)fst01024734 |
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Analysis. |
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fast |
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(OCoLC)fst01079714 |
| 655 #2 - |
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Laboratory Manuals. |
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Electronic books. |
| 776 08 - |
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Print version: |
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Westermeier, Reiner. |
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Proteomics in practice. |
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2nd ed. |
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Weinheim : Wiley-VCH ; Chichester : John Wiley [distributor], 2008 |
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9783527319411 |
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3527319417 |
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(OCoLC)195752789 |
| 856 40 - |
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Wiley Online Library |
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92 |
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DG1 |
