Protein engineering in industrial biotechnology / edited by Lilia Alberghina.

Machine generated contents note: Preface vii -- Contributors ix -- 1 Protein Engineering in Basic and Applied Biotechnology: A Review 1 -- Lilia Alberghina and Marina Lotti -- 2 Enhanced Recovery and Folding of Recombinant Proteins Using -- Fusion Protein Strategies 23 -- Sophia Hober and Matlias Uhlen -- 3 Protein Engineerinng for Afftriity Pf-ifcation: Thl Strep-tag 41 -- Thomas G.M. Schmia. and Arne Skerra, -- 4 Candida rugosa Lipases: From Molecular'Evolution Analysis to the -- Design of a Synthetic Gene 63 -- Marina Lotti and Lilia Alberghina -- 5 Structure-Function Studies on Cutinase, a Small Lipolytic Enzyme -- with a Water Accessible Active Site 75 -- Sonia Longhi, Anne Nicolas, Christian Jelsch and Christian Cambillau -- 6 Lipases from Rhizopus Species: Genetics, Structure and Applications 115 -- Uwe T Bornscheuer, Jiirgen Pleiss, Claudia Schmidt-Dannert and -- RolfD. Schmid -- 7 Solvent Engineering Modulates Stereoselectivity of Microbial Lipases 135 -- Enrico Cernia, Cleofe Palocci and Simonetta Soro -- 8 Stabilization of Industrial Enzymes by Protein Engineering 147 -- Guido Grandi, Imma Margarit, Francesco Frigerio, Renzo Nogarotto, -- Giovanna Carpani, Renata Grifantini and Giuliano Galli -- 9 Design of Biotechnologically Applicable Yeast Carboxypeptidase -- Y Mutants with Increased Preference for Charged P1 Residues 159 -- Kjeld Olesen and Klaus Breddam -- 10 Engineering 3-Glycoside Hydrolases 169 -- RA.J. Warren -- 11 Engineering Specificity and Stability in Glucoamylase from -- Aspergillus niger 189 -- Torben P. Frandsen, Henri-Pierre Fierobe and Birte Svensson -- 12 Pectin Modifying Enzymes and Designer Pectins 207 -- Olga Mayans, John Jenkins, Drummond Smith, Kathryn Worboys and -- Richard Pickersgill.