Wellcome

Production of membrane proteins : strategies for expression and isolation / edited by Anne Skaja Robinson.

Contributor(s): Robinson, Anne Skaja, 1966- [editor.] | Wiley InterScience (Online service)Material type: TextTextPublisher: Weinheim : Wiley-VCH, 2011Description: 1 online resource (xxi, 420 pages)Content type: text Media type: computer Carrier type: online resourceISBN: 9783527634521; 3527634525; 9781283370523; 1283370522; 9783527634545; 3527634541Subject(s): Membrane proteins | Membrane Proteins -- genetics | Membrane Proteins -- isolation & purification | Membrane proteinsGenre/Form: Electronic books.Additional physical formats: Print version:: Production of membrane proteins.DDC classification: 572.696 LOC classification: QP552.M44 | P76 2011NLM classification: QU 55.7Online resources: Wiley Online Library
Contents:
Front Matter -- Introduction / Anne Skaja Robinson, Patrick J Loll -- Expression Systems. Bacterial Systems / James Samuelson -- Membrane Protein Expression in / Zachary Britton, Carissa Young, Ozge Can, Patrick McNeely, Andrea Naranjo, Anne Skaja Robinson -- Expression Systems: / Fatima Alkhalfioui, Christel Logez, Olivier Bornert, Renaud Wagner -- Heterologous Production of Active Mammalian G-Protein-Coupled Receptors Using Baculovirus-Infected Insect Cells / Mark Chiu, Brian Estvander, Timothy Esbenshade, Steve Kakavas, Kathy Krueger, Marc Lake, Ana Pereda-Lopez -- Membrane Protein Expression in Mammalian Cells / Deniz B Hizal, Erika Ohsfeldt, Sunny Mai, Michael J Betenbaugh -- Membrane Protein Production Using Photosynthetic Bacteria: A Practical Guide / Philip D Laible, Donna L Mielke, Deborah K Hanson -- Protein-Specific Considerations. Peripheral Membrane Protein Production for Structural and Functional Studies / Brian J Bahnson -- Expression of G-Protein-Coupled Receptors / Alexei Yeliseev, Krishna Vukoti -- Structural Biology of Membrane Proteins / David Salom, Krzysztof Palczewski -- Emerging Methods and Approaches. Engineering Integral Membrane Proteins for Expression and Stability / Igor Dodevski, Andreas Pluckthun -- Expression and Purification of G-Protein-Coupled Receptors for Nuclear Magnetic Resonance Structural Studies / Fabio Casagrande, Klaus Maier, Hans Kiefer, Stanley J Opella, Sang Ho Park -- Solubilization, Purification, and Characterization of Integral Membrane Proteins / Victor Lorenz-Fonfria, Alex Per̀lvarez-Mar̕n, Esteve Padr̤s, Tzvetana Lazarova -- Stabilizing Membrane Proteins in Detergent and Lipid Systems / Mark Lorch, Rebecca Batchelor -- Rapid Optimization of Membrane Protein Production Using Green Fluorescent Protein-Fusions and Lemo21(DE3) / Susan Schlegel, Mirjam Klepsch, Dimitra Gialama, David Wickstrom, David Drew, Jan-Willem de Gier -- Index.
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Front Matter -- Introduction / Anne Skaja Robinson, Patrick J Loll -- Expression Systems. Bacterial Systems / James Samuelson -- Membrane Protein Expression in / Zachary Britton, Carissa Young, Ozge Can, Patrick McNeely, Andrea Naranjo, Anne Skaja Robinson -- Expression Systems: / Fatima Alkhalfioui, Christel Logez, Olivier Bornert, Renaud Wagner -- Heterologous Production of Active Mammalian G-Protein-Coupled Receptors Using Baculovirus-Infected Insect Cells / Mark Chiu, Brian Estvander, Timothy Esbenshade, Steve Kakavas, Kathy Krueger, Marc Lake, Ana Pereda-Lopez -- Membrane Protein Expression in Mammalian Cells / Deniz B Hizal, Erika Ohsfeldt, Sunny Mai, Michael J Betenbaugh -- Membrane Protein Production Using Photosynthetic Bacteria: A Practical Guide / Philip D Laible, Donna L Mielke, Deborah K Hanson -- Protein-Specific Considerations. Peripheral Membrane Protein Production for Structural and Functional Studies / Brian J Bahnson -- Expression of G-Protein-Coupled Receptors / Alexei Yeliseev, Krishna Vukoti -- Structural Biology of Membrane Proteins / David Salom, Krzysztof Palczewski -- Emerging Methods and Approaches. Engineering Integral Membrane Proteins for Expression and Stability / Igor Dodevski, Andreas Pluckthun -- Expression and Purification of G-Protein-Coupled Receptors for Nuclear Magnetic Resonance Structural Studies / Fabio Casagrande, Klaus Maier, Hans Kiefer, Stanley J Opella, Sang Ho Park -- Solubilization, Purification, and Characterization of Integral Membrane Proteins / Victor Lorenz-Fonfria, Alex Per̀lvarez-Mar̕n, Esteve Padr̤s, Tzvetana Lazarova -- Stabilizing Membrane Proteins in Detergent and Lipid Systems / Mark Lorch, Rebecca Batchelor -- Rapid Optimization of Membrane Protein Production Using Green Fluorescent Protein-Fusions and Lemo21(DE3) / Susan Schlegel, Mirjam Klepsch, Dimitra Gialama, David Wickstrom, David Drew, Jan-Willem de Gier -- Index.

Includes index.

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