TY  - BOOK
AU  - Westermeier,Reiner
AU  - Naven,Tom
AU  - Höpker,Hans-Rudolf
ED  - Wiley InterScience (Online service)
TI  - Proteomics in practice: a guide to successful experimental design 
SN  - 9783527622290
AV  - QP551 .P75673 2008eb
U1  - 572.64 22
PY  - 2008///
CY  - Weinheim
PB  - Wiley-VCH
KW  - Proteins
KW  - Analysis
KW  - Molecular biology
KW  - Proteome
KW  - Proteomics
KW  - SCIENCE
KW  - Life Sciences
KW  - Biochemistry
KW  - bisacsh
KW  - fast
KW  - Laboratory Manuals
KW  - Electronic books
N1  - Previous edition: 2002; Includes bibliographical references and index; Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- pt. III. Trouble shooting -- Glossary of terms
N2  - A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third sectio
UR  - https://doi.org/10.1002/9783527622290
ER  -