Proteomics in practice : a guide to successful experimental design / Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker.
Material type:
TextPublisher: Weinheim : Wiley-VCH ; 2008Distributor: Chichester : John Wiley [distributor], 2008Edition: 2nd edDescription: 1 online resource (xx, 482 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9783527622290; 3527622292; 9783527622306; 3527622306Subject(s): Proteins -- Analysis | Molecular biology | Proteome | Proteomics | SCIENCE -- Life Sciences -- Biochemistry | Molecular biology | Proteins -- AnalysisGenre/Form: Laboratory Manuals. | Electronic books.Additional physical formats: Print version:: Proteomics in practice.DDC classification: 572.64 LOC classification: QP551 | .P75673 2008ebNLM classification: 2008 E-811 | QU 25Online resources: Wiley Online Library | Item type | Current library | Call number | Status | Date due | Barcode |
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Ebooks
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Mysore University Main Library | Not for loan | EBJW659 |
Previous edition: 2002.
Includes bibliographical references and index.
Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- pt. III. Trouble shooting -- Glossary of terms.
A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third sectio.
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